Embryo Freezing in IVF Treatment

Procedure & advantages
What is Embryo Freezing?

What is Embryo Freezing?

The procedure of Embryo freezing is done along with fertility treatment in an IVF laboratory.  It allows people to store gametes, reproductive tissue and embryos for later use. The first successful pregnancy resulting from freezing a healthy embryo took place in the 1980s. Since then, many people have frozen embryos and used them later. If your doctor has given option for frozen embryo transfer you must know the risks and advantages.

Embryo freezing is a laboratory procedure which begins with injection of hormones and other medications to stimulate the production of potentially fertile eggs. The eggs are then extracted from the ovaries, either for fertilizing in a lab or for freezing. A person may wish to freeze their eggs. Or, they may wish to use them at once to become pregnant. Fertilisation of eggs with sperm can be achieved either with in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).  Often with in vitro fertilisation (IVF) or Intracytoplasmic sperm injection (ICSI) treatment, there may be good quality embryos left over after embryo transfer. Instead of discarding them, there is the option to freeze them to use in the future. A person can also freeze eggs and sperm, which are not fertilized. Embryos can be frozen to preserve fertility so it may be possible to have a baby at a later date.

When freezing is recommended?

Embryo cryopreservation is useful for surplus embryos during in vitro fertilisation. Patients who fail to conceive may become pregnant using frozen embryos in subsequent cycles. Successful patients in IVF treatment may come back later for frozen embryo transfer to achieve a sibling pregnancy.


Surplus oocytes or embryos resulting from fertility treatments may be stored for oocyte donation or embryo donation to another woman or couple. At times embryos may be created, frozen and stored specifically for transfer and donation by using donor eggs and sperm.

 When the endometrium is not ready for implantation embryos may be frozen for use at later date. This situation arises in patients with thin endometrium or oocyte donation and surrogacy programme.

 There are certain situations like ovarian hyper stimulation when fresh embryo transfer becomes risky for the woman’s health. In this scenario embryos are frozen for later use.

 Young patients undergoing cancer therapy can freeze their eggs, ovarian or testicular tissue for future use. Rare instances where mother is not fit to undergo a pregnancy may freeze embryos. They can come later for embryo transfer when the health issue is resolved.

How do people freeze embryos?


Cryo-preservation or cryo-conservation is a process where cells, tissues or organs are preserved by cooling to very low temperatures. Cryopreservation was applied to humans beginning in 1954 with three pregnancies resulting from the insemination of previously frozen sperm. There are two ways of freezing-


Slow freezing: This involves placing the embryos in sealed tubes, then slowly lowering their temperature. It prevents the embryo’s cells from ageing and reduces the risk of damage. However, slow freezing is time-consuming, and it requires expensive machinery.


Vitrification: In this process, the tissue is frozen after cryoprotection. The embryos are frozen so quickly that the water molecules do not have time to form ice crystals. This helps protect the embryos and increases their rate of survival during thawing. In the laboratory, large tanks filled with liquid nitrogen are available in which embryos are stored. The embryos remain in sealed containers at temperatures of -321ºF. At this temperature, almost no biological processes, such as ageing, can occur.

  The tanks that contain frozen embryos are monitored 7 days a week. Each tank gets a physical inspection daily, looking for problems or signs of problems. The quantity of nitrogen in the tank is assessed as a means of monitoring for a possible slow leak or an impending tank failure. The nitrogen in the tank is topped up daily, since it continuously evaporates at a slow rate.

When to freeze embryos?


Not all embryos are suitable for freezing, so only good quality embryos will be chosen to freeze. Embryos can be frozen at different stages of their development – when they’re just a single cell, at the two to eight cell stage or later in their development (called the blastocyst stage). Eggs and sperm can also be frozen with out fertilising.


What is the success rates of thawing frozen embryos?


The process of thawing an embryo after cryopreservation has a relatively high success rate, and research suggests that women who use thawed embryos have good chances of delivering healthy babies.


How long can embryos stay frozen?


Procedures for human embryo freezing were developed in 1984 and only went into widespread use in the late 1980s. This means that the longest time a human embryo has been stored is 25-30 years and, typically, patients that have left embryos in storage for this long are not coming back for them.

In theory, a correctly frozen embryo can remain viable for any length of time. But different laws regulate length of freezing in different countries. Most countries allow freezing up to 10 years.


The embryos remain in sealed containers at temperatures of -321ºF. At this temperature, almost no biological processes, such as aging, can occur.


Frozen or fresh embryos – which is better?


A study published in the International Journal of Reproductive Biomedicine looked at the results of over 1,000 instances of embryo transfer involving either fresh or frozen embryos.

The researchers found no statistical difference between the types of embryos, in terms of pregnancy rates or fetal health.


Freezing of human sperm, eggs and embryos @ Femelife


Within the laboratory at Femelife we have a tissue bank where we freeze and store oocytes (eggs), sperm and embryos for our patients. The bank has personnel and computerised system for full time monitoring. The procedure is done under supervision of the lab director, and is licensed by the ICMR, Govt of India. All tissue in the bank is stored frozen in liquid nitrogen at a temperature of -196C in vacuum lined tanks that are computer controlled and monitored 7 days a week with a dedicated alarm system. The embryologists are responsible for maintaining the bank and no other employee has access to it.


The process of freezing eggs vs. freezing embryos


The egg freezing procedure and the embryo freezing procedure both start the same basic way with hormone medication, injected over for 8–12 days, that stimulates the ovaries to produce multiple eggs. Freezing multiple eggs increases the chances of finding healthy eggs later. Study have shown that embryos survive the freeze thaw process better than eggs. The freezing of eggs is a recent procedure and may need further development for wide usage.


What are the risks associated with cryopreservation of embryos and human gamete?


Risk of contamination

 Since viruses and bacteria can also survive at cryogenic temperatures it needs special attention. The risk of cross-contamination, i.e., transfer of bacteria or other microorganisms from liquid nitrogen to stored samples, is should be taken care. Some investigators have suggested that liquid nitrogen should be sterilised in order to prevent such contamination when so-called open cryodevices, which bring the sample in direct contact with the liquid, are utilised.

 Risk of reduced or lost viability

 Long-term storage does not impact viability/developmental potential of slow-frozen embryos. Cryostorage appears to be safe for slow-frozen oocytes as well.


Risk of specimen loss

 In contrast to the risk of infection or time-related decrease in viability, under current practices the risk of loss of cryopreserved gametes and embryos due to human error or equipment failure is relatively high. It is the responsibility of the IVF laboratory to take proper care of the procedure.

 Risks inherent in shipment and handling

 At times, frozen eggs or embryos need transportation for further use according to patient convenience. The shipping procedure carries risk of vessels being exposed to elevated ambient temperature and air pressure, vibration/other physical shock, and horizontal storage.

 A two-step removal of vitrified samples, during which the sample is held in the neck of a dewar in nitrogen vapour for 15 seconds, leads to reduced viability due to thermodynamic instability and devitrification at relatively low temperatures. Cross country transportation of cryopreserved eggs and embryos are guarded by certain laws.

        Embryo freezing is a type of fertility preservation. It may be useful for women with cancer who want to have children after having radiation therapy, chemotherapy, or certain types of surgery, which can cause infertility. Also, called embryo banking and embryo cryopreservation it is widely used in IVF treatment.


Femelife Fertility

since 2010

Contact Us

Consult Our Specialists To Know More